A new enzymatic technology to perform versatile bioconjugation reactions.
Demonstrated across
Enzymatic precision and efficiency for your bioconjugation experiments.
Most site-specific methods pay for precision with long tags, costly custom substrates, or fussy chemistry. Enzura uses a proprietary enzyme to attach a bioorthogonal handle at a short peptide motif.
Site-specific by design
The enzyme recognizes a short peptide motif and installs the handle at one defined position — at the N-terminus, C-terminus, or an internal loop.
Programmable tag
A short, flexible recognition motif you can place where you need it — minimal modification, easily introduced by design.
One-pot, low-cost substrates
Click substrates are generated enzymatically from NAD⁺ (~$1/g) and off-the-shelf alcohols — no expensive custom synthesis, no intermediate purification.
Quantitative small-molecule labeling
Full conversion attaching biotin and fluorophores via CuAAC and SPAAC click chemistry, with modified proteins isolated in good yield.
Stable, minimal scar
The phosphodiester linkage resisted phosphatases and nucleases in testing; after the click reaction only a triazole and a naturally-occurring phosphoribose remain.
Beyond small molecules
We've demonstrated protein–protein and protein–DNA conjugation and covalent immobilization on resin — early, unoptimized, and an active area of our work.
Three steps, one pot reaction.
Make the click substrate
ADP-ribosyl cyclase (ADPRC) converts NAD⁺ and a simple azide- or alkyne-alcohol into a clickable dinucleotide — no purification needed.
Install the handle
our proprietary enzyme transfers the bioorthogonal handle onto the target protein's short recognition tag, site-specifically.
Conjugate your payload
Standard CuAAC or SPAAC click chemistry joins your molecule of choice — dye, biotin, DNA, another protein, or a surface.
Make precise conjugation simple, cheap, and accessible.
Precise bioconjugation shouldn't require long tags, bespoke substrates, or specialist chemistry. ADDing started as a way to change that — an enzymatic route to click handles using materials any laboratory can buy.
- Today — site-specific labeling, biotinylation & immobilization
- Next — higher-yield protein–protein & payload conjugation
- Goal — a low-immunogenicity ADC conjugation platform
Let's build the bond together.
We're looking for research partners, collaborators, and investors who want to bring simple, precise conjugation to real applications.
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