Enzymatic bioconjugation

Site-specific bioconjugation,made simple.

Small tag
Compatible with N-terminal, C-terminal or internal loop
Full conversion
Quantitative handle installation on tested proteins
Low-cost inputs
Bulk, off-the-shelf reagents, no custom synthesis

Applications

Fluorescent labelingCy3 azide on an alkyne-tagged protein: saturated within 20 min by in-gel fluorescence.
BiotinylationQuantitative biotin-PEG4 attachment in 10 min.
Protein immobilizationCovalent capture on commercial azide agarose; withstands very high imidazole wash.
Protein & DNA conjugationN-to-N, C-to-C and branched protein fusions, plus coupling to a 45-nt azido oligo.
The technology

Enzymatic precision and efficiency for your bioconjugation experiments.

Most site-selective labelling methods require long tags, costly custom substrates, or demanding chemistry.

Protein of interest Small tag, any site enzymatic step Handle installed At the chosen site click Payload attached dye · biotin · DNA · protein
Substrate synthesis and handle installation run together in one pot.

Site-specific by design

Programmable tag

Works at the N-terminus, the C-terminus or in an internal loop.

One-pot reaction with low-cost substrates

The click-ready substrate is made enzymatically from bulk, off-the-shelf reagents. No custom synthesis, no specialist supplier, no lead time.

Stable bond, minimal scar

A phosphodiester linkage that remains intact following phosphatase and nuclease treatment across a broad range of pH values, temperatures, and buffer additives, with testing conducted in both plasma and serum.

How it works
01 · SYNTHESIZE

Make the click substrate

A single enzymatic step converts readily available starting materials into a substrate ready for click chemistry. No organic synthesis, custom reagents, or protecting groups required.

02 · BOND

Install the handle

Our enzyme recognises a short tag on the targeted protein and installs the handle at one defined position, with no modification elsewhere in the target of interest.

03 · CLICK

Conjugate your payload

Use the installed handle to attach any compatible partner, from dyes and biotin to oligonucleotides, tagged proteins, and functionalised resins.

Our mission

Make precise conjugation simple, cheap, and accessible.

Precise bioconjugation shouldn’t depend on long protein tags, custom substrates, or specialized chemistry. Enzura Biotech aims to change that.

Let's build the next bioconjugate, together.

Or email us directly at info@enzurabiotech.com